Violence In Entertainment Essays - Dispute Resolution, Criminology

Violence In Entertainment Violence In Entertainment And Its Effect On Society Does entertainment influence society's attitude towards violent behavior? In order to fully answer this question we must first understand what violence is. Violence is the use of one's powers to inflict mental or physical injury upon another, examples of this would be rape or murder. Violence in entertainment reaches the public by way of television, movies, plays, and novels. Through the course of this essay it will be proven that violence in entertainment is a major factor in the escalation of violence in society, once this is proven we will take all of the evidence that has been shown throughout this paper and come to a conclusion as to whether or not violence in entertainment is justified and whether or not it should be censored. Television with its far reaching influence spreads across the globe. Its most important role is that of reporting the news and maintaining communication between people around the world. Television's most influential, yet most serious aspect is its shows for entertainment. Violent children's shows like Mighty Morphin Power Rangers and adult shows like NYPD Blue and Homicide almost always fail to show human beings being able to resolve their differences in a non-violent manner, instead they show a reckless attitude that promotes violent action first with reflection on the consequences later. In one episode of NYPD Blue three people were murdered in the span of an hour. Contemporary television creates a seemingly insatiable appetite for amusement of all kinds without regard for social or moral benefits (Schultze 41). Findings over the past twenty years by three Surgeon Generals, the Attorney General's Task Force on Family Violence, the American Medical Association, the National Institut e of Mental Health, the American Psychiatric Association, the American Psychological Association, the American Academy of Pediatrics, and other medical authorities indicate that televised violence is harmful to all of us, but particularly to the mental health of children (Medved 70-71). In 1989 the results of a five year study by the American Psychological Association indicated that the average child has witnessed 8,000 murders and 100,000 other acts of violence on television by the time he or she has completed sixth grade. In further studies it was determined that by the time that same child graduates from high school he or she will have spent 22,000 hours watching television, twice as many hours as he or she has spent in school (Bruno 124). In a study by the Centers for Disease Control, published by the JAMA (Journal of the American Medical Association), it was shown that homicide rates had doubled between the introduction of television in the 1950's and the end of the study in 1994. In that same study other possible causes for the vast increases in violence were studied, the 'baby boom' effect, trends in urbanization, economic trends, trends in alcohol abuse, the role of capital punishment, civil unrest, the availability of guns, and exposure to television(Lamson 32). Each of these purported causes was tested in a variety of ways to see whether it could be eliminated as a credible contributor to doubling the crime rate in the United States, and one by each of them was invalidated, except for television. Children average four hours of television per day, and in the inner city that increases to as much as eleven hours a day, with an average of eight to twelve violent incidents per hour. It is also interesting to note tha t violence occurs some fifty-five times more often on television than it does in the real world (Medved 156). FBI and census data show the homicide arrest rate for seventeen-year-olds more than doubled between 1985 and 1991, and the rates for fifteen-and sixteen-year-olds increased even faster. Movies also add their fair share to the problem of violence in society. Researchers have established that copycat events are not an anomaly. Statistically-speaking, they are rare, but predictable, occurences. Television shows, novels, but especially movies-all can trigger copycat violence (Medved 72). As recently as November of 1995, New York City officials believed that the burning of a toll-booth clerk was a result of copycat violence, resulting from a similar scene in the movie Money Train. In 1994,

T. Roosevelt, a legacy essays

T. Roosevelt, a legacy essays The Presidency of Theodore Roosevelt The turn of the century has always been a big deal for modern civilizations. One hundred years of life is quite large compared with the average 70 or so given to most. Because of that, people tend to look in trends of decades, rather than centuries or millennia. When it does come time for a new century, when that second digit rotates, as it does so seldom, people tend to look for change. Events tend to fall before or after the century, not on top of it, and United States history, particularly, has had a tendency for sudden change at the century marks. Columbus' accidental discovery of the West Indies in 1492 brought on the exploration age in the 1500s. Jamestown colony, founded in 1607, was England's first foothold on the New World. A massive population surge, brought on in part by the import of fricans, marks entry into the 18th century. Thomas Jefferson's presidency, beginning in 1800, changed the face of American politics. 1900 was a ripe year for change, but needed someone to help the change arrives. That someone was Theodore Roosevelt. Roosevelt's political presence altered the course of the United States, transforming it into a superpower fully ready to handle the challenges of any opposition, and changed the role of the president and executive branch of US government, making it a force with As the first president with progressive views, Roosevelt enacted the first regulatory laws and prosecuted big businesses who had been violating them and others for years. Roosevelt also initiated the United States' active interests in other countries, and began to spread the benefits of democracy throughout the world. Before Roosevelt, the United States was an inward-looking country, largely xenophobic to the calls of the rest of the world, and chiefly concerned with bettering itself. As one critic put it, "Roosevelt was the first mode...

Virtual Workshops Essay Example | Topics and Well Written Essays - 1750 words

Virtual Workshops - Essay Example My formal categories were designed to show frequency in the video that highlighted rage and hostility and frequency of video which illustrated sympathy or disturbed reactions to the verdict. The content analysis format allowed me to establish the precise number of times that the news organization opted to illustrate any of these factors. One of my categories dealt with the social status of the trial participants (career, relationship to Saddam, etc.), which achieved no results in trying to determine whether people varying social status held differing opinions about the death sentence. Other than Saddam and the presiding judge, I could make no determination about social status as the media opted not to offer background information regarding the other trial participants. This failure could be remedied in further analyses by formulating categories for research that can be more readily displayed in the chosen data used for observation. In terms of future research, I might suggest that us ing the category of "intensity" might offer further data regarding whether the instances of emphasizing a media agenda are witnessed in terms of how media interactions take place. For instance, facial grimaces or smiles (in this case applying to the Saddam verdict) and their frequency within a form of media might suggest the emphasis being placed on fulfilling a biased media agenda. Virtual Workshop 3: Observation This is a content analysis report based on the observation carried out on people using a public transport.

Public speaking Essay Example | Topics and Well Written Essays - 1000 words

Public speaking - Essay Example The two goals contradicted each other, as Vietnams had threatened to attack US if their troops were not to be withdrawn from South Vietnams. For that reason, Nixon addressed the issue with a two sided mind. Paradoxically, Nixon imparts a withdrawal mode to his audience, a strategy that makes them feel like he cares so much for the Americans, and especially the American men in Vietnam. He acknowledges that American troops stand a higher risk in the attacked zones but concludes the sentence by mentioning that withdrawing implies a greater risk to the natives of the attached zones. Nixon considers it wise to analyse the situation in two ways to make the audience understand that withdrawal, as the solution suggested earlier, is a controversial action that worsens the situation. As matter of fact, the Nixon understands that his audience are Americans, who would want their men protected but keeps in mind that the outside world is also watching his action. Therefore, the speech is planned n ot only to please the immediate audience but ensure that the problem is effectively addressed. Nixon confidently convinces the audience that his speech is well advised, by mentioning that the decision being presented wasn’t his own opinion but an informed discussion with national Security Council, other crucial personnel as well as the president’s advisers. To ensure this confidence, the speech creates some sense of inductive reasoning, by first defining the problem, analysing the available solutions and drawing a generalised conclusion later on. Nixon explains the problem by describing the actions and motives of the enemy. According to Nixon, America has no enmity whatsoever with North Vietnam, in the past there had been no troops moving to attack Vietnam, neither had the south Vietnams attacked their opponents before . He at the same time mentions the existence of alliance with South Vietnams. These create some sense of reasoning to the audience that

Malcolm Gladwell's David and Goliath presentation Assignment

Malcolm Gladwell's David and Goliath presentation - Assignment Example David’s brand is an underdog in this presentation that serves to show the upper hand he had against Goliath. Contextually, Gladwell argues that nimble, newcomers with new answers to old challenges frequently beat giant risks or barriers. David’s strong suit originated from his ability to break the presumptions of conventional battle strategies that size means power.2 Here, Gladwell says that being the underdog creates a situation that shows why there is always more than meets the eye. Using context to express an analysis of the account of David and Goliath makes Gladwell’s work convincing.3 The presentation is essentially about spiritual weapons as well as how affective and imaginative desires are as equally important as financial or material desires. In the absence of physical advantages, one has ideas, imagination, perseverance, devotion, and excitement to keep going. A critical look at the story of David and Goliath underscores this near rationale as I was convinced to appreciate my ideal and imaginative gifts as they are, as should any other historian or even

Effect of Mutant EDA-A1 Gene on Huvecs

Effect of Mutant EDA-A1 Gene on Huvecs Effect of EDA-A1 gene mutant on proliferation and cell cycle distribution of cultured human umbilical vein endothelial cell Running title: The effect of mutant EDA-A1 gene on HUVECs. Ke Lei, MM; Lunchang Wang, MD; Bing Ma, MM; Ping Shi, MD; Longjiang Li, MD; Tuanjie Che, MD; Xiangyi He, MD Highlights: EDA-A1 gene mutant significantly decreased proliferation of human umbilical vein endothelial cells (HUVECs). HUVECs of mutant group were blocked at G0/G1 and S phase. HUVECs of wild group accumulated in S phase and decreased in G2/M phase. Abstract Background: To investigate the effect of ectodysplasin A gene (EDA-A1) on proliferation and cell cycle of human umbilical vein endothelial cells (HUVECs) and explore the possible mechanism underlying this process. Methods: Recombinant eukaryotic expression vectors pcDNA3.1(-)-EDA-A1-M/W (mutant, M; wild, W) containing the coding sequence of EDA-A1-M/W were transfected into HUVECs. EDA-A1-M/W genes were amplified by reverse transcription polymerase chain reaction (RT-PCR), and the proteins were detected by western blot. Then MTT assay for cell proliferation of HUVECs in each group was performed and cell cycle was detected using flow cytometry. Results: The EDA-A1 gene and protein were detected respectively by RT-PCR and western blot in HUVECs transfected with pcDNA3.1(-)-EDA-A1-M/W, but not in HUVECs transfected with empty plasmid pcDNA3.1(-) (control group) and cells without transfection. Compared with control group, EDA-A1 gene mutant significantly decreased proliferation of HUVECs and the inhibition rate was 45.70% (PEDA-A1 gene did not cause such growth inhibition (P>0.05). A significant increase of the G0/G1 and S fraction was seen in the HUVECs of mutant group, compared with wild group with an accumulation in S phase and a concomitant decrease in G2/M phase population (P Conclusion: Compared with the wide-type, the mutant EDA-A1 gene could inhibit the proliferation and cell cycle of the HUVEC. Key words: EDA-A1 gene; Mutant; Human umbilical vein endothelial cell; Cell cycle; Proliferation Introduction Hypohidrotic ectodermal dysplasia (HED), also called anhidrotic ectodermal dysplasia (AED) or Christ-Siemens-Touraine Syndrome, is a kind of X-linked recessive genetic disease (XLHED) (1). HED is a rare congenital genetic disorder with a birth incidence of 1/100,000-1/10,000 (2, 3). It is characterized by the diminution or absence of eccrine sweat glands, oligodontia and peg shaped teeth and sparse hair (1, 4). Previous study indicates that XLHED is caused by the ectodysplasin A gene (EDA-A1) mutant (5). EDA-A1, a major causative gene of HED, locates in Xq12-13.1 and encodes a novel tumor necrosis factor (TNF) ligand family protein ectodysplasin A (EDA-A1) and this protein is associated with the nuclear factor-ÃŽ ºB (NF-ÃŽ ºB) signaling mechanisms (5-9). Bayes M et al. (10) indicates that the full-length of EDA-A1 is 5296bp (http://www.ncbi.nlm.nih.gov/, AH007059, Gene ID 4007891), the open reading frame (ORF) of EDA-A1 is 1176bp, and it encoding the protein with 391 amino acids (EDA-A1, GeneID1896). Studies showed the combination of EDA-A1 and ectodysplasin receptor (EDAR) could promote programmed cell death and active the signaling of NF-ÃŽ ºB (8, 11). Recently, the related research on HED are mostly for mutation analysis of EDA-A1, and more than 100 mutations in the EDA gene have been reported to cause XLHED up to now (12, 13). However, there have few reports relating to the function of mutant EDA-A1, and the exact pathological mechanism of mutant EDA-A1 on HED is still unclear. In the present study, EDA-A1 mutant (pcDNA3.1 (-)-EDA-A1-M) and wild type (pcDNA3.1(-)-EDA-A1-W) eukaryotic expression vector that we used were constructed in our previous study (14). Then the function of transfected EDA-A1 and its mutant for cell proliferation and cell cycle of HUVECs were analyzed. The aim of this study was to investigate the effect of EDA-A1 on proliferation and cell cycle of HUVECs and explore the possible mechanism underlying this process. Material and Method Cell culture HUVECs were kindly provided by professor Wang chunming (Lanzhou University, China). HUVECs were cultured in RPMI-1640 (Huamei Company, Shanghai, China) Medium. The medium were consisted of 10% fetal bovine serum (FBS) (Evergreen Company, Hangzhou) and 100U/ml penicillin/streptomycin. All these cells were maintained in humidified incubator of 5% CO2 at 37à ¢Ã¢â‚¬Å¾Ã†â€™ (0.25% trypsin digestion overnight). Inverted microscope was used for the cell morphology investigation. All the experiments were performed at least in triplicate and repeated at least twice. Plasmid extraction EDA-A1 mutant (pcDNA3.1(-)-EDA-A1-M) and wild type (pcDNA3.1 (-)-EDA-A1-W) eukaryotic expression vector that we used were constructed in our previous study (14). Totally 3ÃŽ ¼l mutant (M) and Wild-type (W) plasmid DNA was extracted respectively from transfected HUVECs, followed by the sterile deionized water diluted to 1ml. The values of à ¢Ã¢â€š ¬Ã¢â‚¬ ¹Ãƒ ¢Ã¢â€š ¬Ã¢â‚¬ ¹A260nm and A280nm were measured by UV spectrophotometer. Plasmid DNA concentration (ÃŽ ¼g / ÃŽ ¼l) = A260 Ãâ€" dilution factor Ãâ€" 50/1000. The plasmid DNA (positive recombinants and empty control) was precipitated by ethanol. Then the DNA pellet was resuspended in sterile deionized water. Cell transfection Cell transfection was carried out according to the instructions of QIAGEN-Effectene Transfection Reagent Kit (QIAGEN). Transfection was carried out when the cell density was up to 70% after 24 hour-cell passaging. Cells were transferred into a complete medium (CM) 2 hours before transfection. Totally 2.5 µg mutant (M) and Wild-type (W) plasmid DNA was slowly added to the 2 M CaCl2 solution (stand for 10 minutes). DNA-CaCl2 solution was slowly added dropwise to the 2 Ãâ€" HeBS (stand for 30 minutes) until the precipitation of tiny particles. The precipitate was uniformly dropwise added to the culture flasks. After a 12 hours growth under standard conditions, cells were washed 2 times with HeBS, followed by the cultured in CM. HUVECs transfected with empty vector were used as the control group. Semi-quantitative real-time PCR To identify the expression levels of EDA-A1 in HUVECs, semi-quantitative real-time PCR (SqRT-PCR) analysis was performed. Total RNA was extracted from cultured cells in each group (cultured for 48 hours) by using reverse transcription (RT) kit (Fermentas Company), followed by the EDA-A1 primers designation (Primer Premier 5.0 software) and synthesis (Shanghai Biological Engineering Company ). The primers used were as follows, EDA-A1 (408bp): 5’- CGC AGG ATC CAT GGG CTA CCC GGA GGT -3’ (forward) and 5’- ATT AAG CTT GCC AAG CGG GCA CCA GGG AGA C -3’ (reverse), ÃŽ ²-actin (230bp): 5’- ACG CAT TTG GTC GTA TTG GG-3’ (forward) and 5’- TGA TTT TGG AGG GAT CTC GC-3’ (reverse). The 50ÃŽ ¼l PCR reaction system were: cDNA template (2ÃŽ ¼l), 10 Ãâ€" PCR Buffer (5ÃŽ ¼l), dNTP (1ÃŽ ¼l), primer (up and downstream, 1ÃŽ ¼l), Taq DNA polymerase (1ÃŽ ¼l), ddH2O (39ÃŽ ¼l). Products were subjected to electrophoresis (1.5% agarose gel, 120V, 90mA). Western blot analysis For Western blot analysis, proteins were extracted from HUVECs in each group. Proteins were collected after cell lysis. Protein concentration was determined using the Bradford dye-binding method (15). The proteins were separated by SDS-PAGE and transferred to the 0.45ÃŽ ¼m pore size nitrocellulose (NC) membrane (RPN303E, Amersham Company). NC membranes were blocked with TBS buffer (5% milk and 0.5%-Tween) for 1 hour (37 °C). Then, the membrane was incubated overnight at 4à ¢Ã¢â‚¬Å¾Ã†â€™ with the rabbit antibodies EDA-A1 and ÃŽ ²-actin (1:200 dilution with TBST solution), followed by incubation at room temperature for 1h with an anti-rabbit secondary antibody (Sigma). Finally, the expression levels of the target proteins were visualized withchromogenic substrate. MTT assay for cell proliferation detection To determine the proliferation of HUVECs in each group, the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay was performed. The 24 hours-transfected and untransfected cells were seeded into 96-well plate with inculation density of 5000 cells/well and incubated at 37à ¢Ã¢â‚¬Å¾Ã†â€™. After 12 hours, 100 ÃŽ ¼l serum-free DMEM was added in each well. After 72 hours, 20 ÃŽ ¼l MTT was added into each well to continue incubation at 37à ¢Ã¢â‚¬Å¾Ã†â€™(4 hours). Then, the medium was removed and the precipitation was dissolved in DMSO. The absorbance at 560 nm was measured by SpectraMax 190 microplate reader (Moteular Devices Company) for colorimetric analysis. Inhibition rate of cell growth was calculated (n=10) based on the experimentally measured absorbance value (OD value). Cell cycle analysis Flow cytometry was used to detect the cell cycle.After incubation for 48 h, the cells were collected and washed with cold PBS. The washed cells were fixed in 70% cold ethanol with incubation overnight at 4à ¢Ã¢â‚¬Å¾Ã†â€™. To stain the cells, prodium iodide (PI) solution was added. Flow cytometer (Coulter Epics XL, Beckman Coulter Company) was used to analyze the samples. Cell Quest software was used to analyze the cell percentage of G0 / G1 phase, S phase, and G2 / M phase. Statistical analysis All assays were performed in triplicate and datawere expressed as mean values  ±s.d. The SPSS 13.0 software employing ANOVA was used to analyze all data which expressed as mean ±SD. P values less than 0.05 was considered as significantly different. Results EDA-A1 expression pattern in HUVECs influenced by plasmid-mediated transfection To identify the expression level of ED1-A1 in HUVECs transfected with vector pcDNA3.1(-)-EDA-A1-M or pcDNA3.1(-)-EDA-A1-W, the RNA samples with an OD260/OD280 ration of 1.8-2.0 were chosen for RT-PCR. The HUVECs with pcDNA3.1(-)-EDA-A1-M or pcDNA3.1(-)-EDA-A1-W transfection showed a band nearly 400 bp compared with control using semi-quantitative PCR and primers specific to EDA-A1 (Figure 1). Additionally, ÃŽ ²-actin band between 200 bp and 300 bp have been seen in all the groups. Then, EDA-A1 protein expression in HUVECs were detected by western blot. Figure 1 shows that the EDA-A1 protein was expressed in the transfected cells with pcDNA3.1(-)-EDA-A1-M or pcDNA3.1(-)-EDA-A1-W vector, however, it could not be achieved in control group. In conclusion, the EDA-A1 was expressed in HUVECs after exogenous delivered of EDA-A1, but not in the un-treated control cells. Overexpression of EDA-A1 affects HUVECs proliferation To elucidate the effect of EDA-A1 on HUVECs proliferation, the MTT assays were performed. As shown in Figure 2, the HUVECs viability at 96 h transfection was decreased significantly in the mutant group by comparison with wild type and control. The proliferation of mutant group cells was suppressed by 45.7% compaired to control, while the wild type group was suppressed by 16.0% (Table 1, Figure 3). EDA-A1 overexpression regulates the cell cycle of HUVECs To determine the role of plasmid-mediated EDA-A1 transfection in cell cycle of HUVECs, the flow cytometry was used (Figure 4). We observed that 25.45  ± 1.89 % cells were arrested at G0/G1 phase of cell cycle in the mutant group compared with 20.37  ± 0.6% and 20.30  ± 0.68% cells in wild type and control groups, respectively (Table 2). During S phase, both mutant and wild type groups showed significantly higher cell percentages (14.80  ± 1.45% and 12.4 0  ± 1.75%) than that of control (8.55  ± 0.57%). However, both transfection groups had lower cell percentages than control in G2/M phase. The lowest cell percentage with 62.15  ± 1.94% was showed in the mutant group during S phase. We could conclude that the cell cycle distribution in G0/G1, S, and G2/M of HUVECs were regulated by EDA-A1 overexpression. Discussion HED characterized by impaired development of hair, eccrine sweat glands and teeth is caused by mutations in the EDA-A1 gene (3, 16). Recently, the related research on HED are focused on the mutation analysis of EDA-A1, however, the exact pathological mechanism of HED caused by mutant EDA-A1 is still unclear (17). In this study, we investigated the effect of HED related gene EDA-A1 on proliferation and cell cycle of HUVECs. The results showed that mutant EDA-A1 gene significantly decreased proliferation of HUVECs (P EDA-A1 protein, a type à ¢Ã¢â‚¬ ¦Ã‚ ¡ transmembrane protein, is one of the TNF ligand family members involved in ectodermal development (18). EDA-A1 contains a TNF-like domain (aa: 245–391), a collagen domain, and a furin protease recognition sequence (7, 8, 19-21). The TNF-like domain is necessary and sufficient for receptor molecule EDAR binding (22, 23). Furthermore, EDA-A1 has been shown to specifically bind to EDAR, which could promote programmed cell death and active the signaling of NF-ÃŽ ºB (8, 11). In our study, the reason why EDA-A1 mutant could inhibit the proliferation and block the cell cycle progression in G0/G1 phase and S phase of HUVECs might be the change of protein spatial configuration and biological activity that caused by the EDA-A1 gene mutation and the changed protein could not combined with EDAR and thus inhibit the signaling of NF-ÃŽ ºB. Maria et al. found that HED was related with the blocked signaling pathway of NF-ÃŽ ºB (9). Pascal et al. found th at point mutations in the TNF-like domain of EDA-A1 strongly decreased EDAR binding to EDA-A1 by altering the folding of EDA (21). Moreover, the substitution of Gln306 with Pro in our study was found to be located in the TNF-like domain of EDA-A1 and may influence the epithelial signaling pathway required for the normal ectodermal development through altering the topology of EDA, which is consistent with previous study. HUVECs are cells derived from the endothelium of veins from the umbilical cord, and they are often used as a laboratory model system for the study of the function and pathology of endothelial cells (24). Some studies showed that during vascular development and pathological angiogenesis, the maintenance of blood vessel homeostasis and its functional execution depend on the integrity of vascular endothelium, which is affected by proliferation, migration and apoptosis of endothelial cells (25, 26). Furthermore, Jie et al. showed that recovery of injured endothelial cells through regulated endothelial cell proliferation plays significant roles in thrombosis disease (27). In our study, mutant EDA-A1 decreased the proliferation of HUVECs, therefore, we suspected that pathological mechanism underlying HED caused by EDA-A1 may be the growth inhibit of endothelial cells which could lead to the defection of eccrine sweat glandsis. Despite of all results mentioned above, there were still some l imitations in the present study, whether the EDA-A1 mutant blocked the combination of EDA-A1 with EDAR required further experiment. In conclusion, our study revealed EDA-A1 gene mutant could inhibit the proliferation and cell cycle of HUVECs. We explored the mechanism of HED caused by mutant EDA-A1. The substitution of Gln306 with Pro may influence the epithelial signaling pathway required for the normal ectodermal development through altering the topology of EDA, which could impair the binding of EDA-A1 to EDAR and further inhibit the signaling of NF-ÃŽ ºB. Our finding broadens the spectrum of EDA-A1 mutations and may help to understand the molecular basis of XLHED and aid genetic counseling. Acknowledgements We wish to express our warm thanks to Fenghe(Shanghai) Information Technology Co., Ltd. Their ideas and help gave a valuable added dimension to our research. Conflict of interest The authors have declared that no competing interests exist. Authors’ contributions KL and LW participated in the design of this study, and they both performed the statistical analysis. BM and TC carried out the study, together with PS, collected important background information, and drafted the manuscript. LL and XH conceived of this study, and participated in the design and helped to draft the manuscript. All authors read and approved the final manuscript. Figure legends: Figure 1 Detection of mRNA expression of EDA-A1gene in ECV304 cells by RT-PCR: M: mutant group; W: wild group; C: control group. Figure 2 Expression of ECV304 cells transfected with EDA-A1 gene and mutant: M: mutant group; W: wild group; C: control group. Figure 3 OD560 value of ECV304 cells transfected with EDA-A1 gene after cultured for 96h: M: mutant group; W: wild group; C: control group; a: compared with the control group, P Figure 4 The effect of EDA-A1 gene mutant on cell cycle in ECV304 cells. Table 1 OD560 value of ECV cells transfected with EDA-A1 gene after cultured for 96h Note: a: compared with control group, P Table 2 Effect of EDA-A1 gene mutant on cell cycle in ECV304 cells Note: a: compared with control group, P

Consider the theme of loneliness in Of Mice and Men. How does it Essa

Consider the theme of loneliness in 'Of Mice and Men'. How does it affect the friendships and relationships in the novel? This novel that was written by John Steinbeck which was set in the 1930s in Salinas Soledad which is in California. The novel consists of many historical factors which have affected the characters in this novel and one of them includes "the great depression" Which leads the novels inspiration for the famous writer John Steinbeck which he mainly based on his own experience. In those days people travelled a lot differently to how we travel now. In those days migrant workers travelled extravagant distances looking for a job. There are many different themes in which are based throughout the whole book, such as loneliness, happiness, nature, dreams and reality: - Which even lead to catastrophe. Many of the people in this novel have very lonely lives mainly because they are migrant workers and as we know they don't have time to make any friends or have any time to spend with their families. There are many characters that are lonely due to age, sex, and race. Two good examples would be Candy because of his age and Crooks because of his race. This novel consists of two main characters George and Lennie, who are an anomalous pair of migrant workers that look after each other. They are completely the reverse of each other. George is the one who has the communicative face and thinks of all of their problems and ideas and tells the other one what to do. Being like this all of the time, in what ever the story it is, always pictures that you would be the small quick one with sharp features. Lennie is the guy that is tall and always does what he is told and has an ill-defined, solid, a... ... gets treated like a little girl. Curley's wife often dreams about herself becoming an actress. At the end of the novel, her loneliness causes Lennie's death. Before Lennie's death, Curley's wife and Lennie were talking in the barn whilst everyone else was playing games. They began talking to each other about each others dreams. They both talked about each others dreams and what they wanted to do in their life. Lennie has a fascination of stroking things. He was stroking Curley's wife's hair, he began to stroke her hair so hard, that he lost control and broke her neck. This has a big impact on George, Lennie and Candy's relationship, as Curley wants to kill Lennie. As a result to this, George has to kill Lennie before he gets killed by Curly. Loneliness will always end in tragedy and dreams will rarely become reality. Friendship never ends.

Automobile †Transport Essay

There are many different kind of transportation vehicles that changed people’s lives; including automobiles, bicycles, airplanes, and many more. I feel automobiles have affected the lives of Americans the most. They have made it possible for individuals to travel many miles a day. Before the automobile, people in cities had to take streetcars to get anywhere farther than they could walk. People who lived out of town had to get to work on trains. There are many different kind of transportation vehicles that changed people’s lives; including automobiles, bicycles, airplanes, and many more. I feel automobiles have affected the lives of Americans the most. They have made it possible for individuals to travel many miles a day. Before the automobile, people in cities had to take streetcars to get anywhere farther than they could walk. People who lived out of town had to get to work on trains. People who lived on farms or in small villages in the country would come into town maybe once a month in a horse-drawn wagon to do their shopping, but a horse couldn’t walk more than few miles. Automobiles provided greater mobility for people. They provided more choices to live. When choosing a job in the old days people tried to find homes that were closest to their work. Cars made it possible for people to move 20-30 miles or more from the city and drive in to work every day. Through my experiences getting to school from bus is a lot slower than cars. I can wake up a lot later if my dad drives me to school. Cars helped transportation become easier and faster. A person could be transported anywhere either by himself or with his family without stopping to rest to feed their horses. Families could sit in comfort while listening to music. However, cars have brought a negative effect on the world, like pollution from exhaust emissions. Cars also take up a lot of space including parking space and parking lots which replace many buildings. It is more dangerous to drive than to walk because of crashes. The ease of a drunken man driving a car is much more dangerous than a drunken man walking. Automobiles can be more dangerous but it provides a lot. Automobiles have had positive and negative effect on the world and changed many people’s lives. Traveling great distances is not much of a problem for people. It also bought time for many people; walking may take 30 minutes but by car it only takes five minutes. Without automobiles the world would be a whole different world. Roads wouldn’t be paved and traveling would be a great deal harder.

The god of small things Essay Example

The god of small things Essay Example The god of small things Paper The god of small things Paper Essay Topic: The God Of Small Things Velutha had worked at the Paradise Pickles and Preserves Factory for a number of years and had been a worker (caste aside) who was appreciated for what he does. He had become an invaluable worker, with Mammachi giving him the accolade that if only he hadnt been a Paravan, he might have become an engineer. [7] I think Roy adopts a more lenient portrayal of Velutha as a Paravan. Mammachi pays him less than a touchable carpenter but more than a Paravan[8]. Veluthas presence is unsettling to many who believe he acts above his station. Veluthas oppression is compounded by the fact that his own father seems to reject his behaviour. : Perhaps it was just a lack of hesitation. An unwarranted assurance. In the way he walked. The way he held his head. The quiet way he offered suggestions without being asked. Or the quiet way in which he disregarded suggestions without appearing to rebel[9]. In the context of this novel, Roy portrays Velutha as someone who doesnt really adopt a different lifestyle simply because he is of an apparent lower class. In simple terms Velutha doesnt adhere to the unfortunate limitations placed upon him to the idea of being of a lower class and he openly acts in a way that implies he doesnt regard him being an inferior person. I dont think that this is ignorance on Veluthas part, simply Roys ability to expose and explore peoples reactions to those who go against the social conventions of the time. Hindus believe that being an Untouchable is punishment for having been bad in a former life. By being good and obedient, an Untouchable can obtain a higher rebirth. Veluthas lack of complacency causes him many problems throughout the novel. It was not entirely his fault that he lived in a society where a mans death could be more profitable than his life had ever been [10]. Although he is a dedicated member of the Marxist Party, his Untouchable status makes other party members dislike him. This idea is comical in its entirety; Velutha is accepted into this party and yet still manages to be denied full recognition as a party member. When Velutha has an affair with Ammu he breaks the rigid love laws, he breaks the rigid social rules of the caste system and therefore he must be punished for his actions. Roy describes the policemens violent actions as being done out of fear, civilizations fear of nature, mens fear of women, powers fear of powerlessness[11]. The division between the Touchables and Untouchables is so ingrained in Kerala society that Velutha is seen as a nonhuman. If they hurt Velutha more than they intended to, it was only because any kinship, and connection between themselves and him, any implication that if nothing else, at least biologically he was a fellow creature- had been severed a long time ago. [11i] Looking at the History House, it demonstrates exactly how those who go against the rules of society are treated. It seems as though the police use force and then hope to answer questions later. Society responds to this act swiftly, with unwavering harshness[12]. Roy doesnt treat the beating of Velutha with dignity or nobility, to which I thought she might, but with gritty detail thud of wood on flesh boot on bone[13]. This grittiness is used by Roy to perhaps contrast with the graceful and enchanted[14] use of vocabulary that goes before. The book as a whole has been acclaimed for its flowery and extravagant use of words and the way theyve been put together. But with this chapter in particular this typical Roy technique subsides to become a more real representation of whats happening. She uses short, sharp sentences to mirror the continual attack of Velutha. Roy makes it clear that the police hold no regard for Velutha, and presents it in such a way that the police feel it is their duty to do what they did, they were merely inoculating a community against an outbreak. [15] I think the image of one policeman flicking at his (Veluthas) penis with a stick[16] expresses his and perhaps societys need to be dominant. This mocking of someones manhood, someone who cant argue back is suggestive of society as a whole where people are oppressed because of their caste, sex, even age. But I think that Roy does go too far in trying to glorify the character of Velutha. Although I think his death was acceptable within the context of the novel, Velutha as a character doesnt seem to fit in with gritty realism and despair of others. Roy liked to use symbolism in the novel and the Untouchable could therefore be more a symbol than a person, necessarily distant from the middle class embroilments that engulf him. [17] It could also be argued that Roy suffers from constraints, that she follows political correctness in the novel and feels compounded to depict the oppressed untouchable without criticism. Similarly the death of Ammu seems too much as if Roy is succumbing to the power of the Indian-woman-as-victim[18]. Limitations placed on females are inherent in Indian society, perhaps all over the world, and Arundhati Roy uses this prejudice to good effect in the novel. Mammachi is a character who is weighed down heavily by male dominance. As the founder of the family pickle factory, this is one of only a few acts of female assertiveness. And yet she is still not given the respect that she deserves, One night Pappachi broke the bow of Mammachis violin[19]. Mammachi runs the business and Whenever anything serious happened in the factory, it was always to Mammachi and not Chacko that the news was brought. [20] But still it is Chacko who assumes the position of the leader of the factory. It seems, inbuilt into the minds of men is the impression that they shall take a dominant role in affairs whether it is their rightful place or not. Chacko arrogantly says My pickles, my jam, my curry powders. [21] However it could be argued that this arrogance is not in Chackos character but merely representative of mens attitudes towards women in India as a whole. Not only is Mammachi contending with her chauvinistic son who frequently uses the women at the factory for sex she also has to contend with her husband, Pappachi. We clearly see the relationship that Mammachi has reluctantly stayed in for many years is wrong. And we find it hard to understand why Mammachi has put up with the beatings for so long. However what Roy is trying to say is that in Indian society leaving your husband is not the done thing, whatever the circumstances. Roy beautifully and yet painfully uses the description of the great grandmothers portrait With her eyes she looked in the direction her husband looked. With her heart she looked away[22] to reveal to the reader the true feelings of Mammachi- a feeling that we as readers suspect to be true but which hadnt been revealed to us. Roy deals with this concept without emotion, which allows the reader to impart the emotion. Perhaps she feels that as English readers we can empathise more with the wife beating because we live in a society that holds no regard for the abuser, whereas Indian readers may be more used to a husband beating a wife, and therefore accept that it happens. Although Roy introduces us to a culture that has many differences to the one that we are part of she does try to make it more accessible to western readers. Now and again Roy will use a minor detail such as television to remind us that this country is not alien. When reading the novel it is hard not to get encapsulated into the unfolding story and at times Kerala does seem like another world. However these minor touches to detail remind us that this is a country that is not so different, albeit that it has differences with respect to traditions. I think Baby Kochammas encounter with the material world is quite amusing- the idea that she has lived her life backwards. As a young woman she renounced the material world, and now, as an old one, she seemed to embrace it. She hugged it and it hugged back[23]. This possibly shows a warmer side to Baby Kochamma or most probably is symbolic of the idea that if someone shows a little love they would receive a little love. This love is evidently missing from the life of Baby Kochamma. For Roy to fully exploit and explain the culture in Kerala would be intensely difficult; and to accomplish it in a novel is simply impossible. We mustnt forget that The God of Small Things is a novel, and that Roy dictates what happens- Velutha is not a real person, Sophie Mol didnt die and probably doesnt exist. But as the reader we are drawn into her [Roys] story and can only be intrigued by the new ways of life introduced to us by Kerala, India. One critic has said that the women in Kerala are relatively free[24]. But as the Western reader we can only take the perspective of how we expect women to be treated in society. It can be argued that we still place limitations on women, yet when presented with the restraints on women in the novel we feel astounded at how confined women are. Roy allows us as readers to make up our own mind about the culture of India; she doesnt simply condemn the caste system or the repression of women. Vulnerability, the ability to be physically and psychologically wounded, appears in this novel as the condition almost all the characters; whether likeable or unlikable, share. I think the role of the bitter and resentful Baby Kochamma is used by Roy to show how people, especially women, can be affected by their surroundings and moulded into the person they become. Roys intentions in writing the novel were probably to move away from the conventional exotic post-colonial novels about the land of heat and dust, incense and spices[25] and impart some gritty realism into Indian society. She shows death, family decay, incest, paedophilia for what they are but intertwined with them is exotic and vibrant language. This antithesis I feel is what makes the novel more tragic because on one hand you are tempted by the bright mangoes Red bananas[26] and on the other are repulsed by the gurgle of blood on a mans breath[27]. It is these stark contrasts that will forever alter the course of the lives of all the members of the family, sending them each off on spinning trajectories of regret and pain. [28] The problem is, its a very sad book and somehow the sadness of the book is what stays with me. [Arundhati Roy][29]. I think the sadness is not simply the deaths of Sophie Mol and Velutha, or the destruction of lives but why these things occur: caused by the unlawful set of laws that dictate the lives of Indian society. Word count: 2567 words References [1] www. freespeech. org/manushi/103/review. html [2] http:/website. lineone. net/~jon. simmons/roy. com [3] The God of Small Things by Arundhati Roy [4] The God of Small Things by Arundhati Roy [5] www.emory. edu/ENGLISH/Bahri/caste. html [6] Mr. Williams English teacher [7] The God of Small Things by Arundhati Roy [8] The God of Small Things by Arundhati Roy [9] The God of Small Things by Arundhati Roy [10] The God of Small Things by Arundhati Roy [11] The God of Small Things by Arundhati Roy [11i] The God of Small Things by Arundhati Roy [12] The God of Small Things by Arundhati Roy [13] The God of Small Things by Arundhati Roy [14] www. londonstudent. org. uk/4issue/arts/godofsmall. htm [15] The God of Small Things by Arundhati Roy [16] The God of Small Things by Arundhati Roy [17] www. freespeech. org/manushi/103/review. html [18] www. freespeech. org/manushi/103/review. html [19] The God of Small Things by Arundhati Roy [20] The God of Small Things by Arundhati Roy [21] The God of Small Things by Arundhati Roy [22] The God of Small Things by Arundhati Roy [23] www. freespeech. org/manushi/103/review. html [24] www. wsu. edu:8080/~brians/anglophone/roy. html [25] www. freespeech. org/manushi/103/review. html [26] The God of Small Things by Arundhati Roy [27] The God of Small Things by Arundhati Roy. [28] www. curledup. com/godsmall. htm [29] Arundhati Roy Bibliography i The God of Small Things by Arundhati Roy i www. freespeech. org/manushi/103/review. html i www. emory. edu/ENGLISH/Bahri/caste. html i www. londonstudent. org. uk/4issue/arts/godofsmall. html i www. wsu. edu:8080/~brians/anglophone/roy. html i www. india50. com/arundhatI. html i www. postcolonialweb. org/india/roy/nishant1. html i endeavor. med. nyu. edu/lit-med/ /webdocs/webdescrips/roy1177-des-. html i www. becal. net/toolkit/damaris/godost. html i www. greenville. edu/~hayes/roy. html.

Code of hammurabi.

Code of hammurabi. Although Hammurabi was probably strong enough to maintain rule through the might of the sword, he provided a set of laws bringing justice and stability to a chaotic and violent region. His laws had the following sections: the prologue, the administration of justice, property, irrigation, loans and interest, regulation of trade, debt slavery, marriage and the family, adoption, personal injury and manslaughter, physician's fees and malpractice, building regulations, wage regulations, and an epilogue. Each line within each section covers something important to the operations of Babylonian society. Although some of Hammurabi's ideas appear harsh, they probably brought stability to this society. The alternative was chaos and war.The most important section of this code is probably the prologue. It shows that these laws do not come from one man. Hammurabi makes it clear that he is the king and wants order and stability in the land. Disobeying the laws means not only violating the wishes of the king, but also angering the gods.An inscription of the Code of Hammurabi.The threat of death appears frequently in these sections. False witness, fraud, and robbery are all ways in which could result in execution of the offender. The simple act of allowing unwanted people to stand within the seller's establishment could prove fatal. However, it is not easy to bring order to a world that has seen little of it ever.Babylon had been settled for centuries, giving its residents time to become concerned over property. Not only is there a specific section devoted to property, but also these concerns appear throughout the code. Property is a major factor in marriages, and concern must be placed on who owns what, especially when the husband pays attention to women other than his wife. The wife has a dowry and sometimes children. The concubine may also produce children competing...

Risk management Assignment Example | Topics and Well Written Essays - 2500 words

Risk management - Assignment Example Integrating risk assessment in every process at the organization improves the quality of decisions made in avoiding losses and eliminating uncertainties. The approach taken in risk management proposes that organizational processes and decisions adopt a thorough implementation of projected objectives. It therefore implies that risk management principles facilitate the integration of every level of coordination of organizational operations and functionalities. Inclusiveness of every organizational need in the success definition elevates the management of the organization to a vintage point enabling tackling of all challenges. Reliance on the latest form of information and of the best available quality enables the risk management concept an important organization development strategy since it enables anticipation and understanding. Accountability to the human society and every vital stakeholder in the projects under the organization ensures that the organization development is in tandem with the aspirations of the stakeholders and to whom accountability is expected. In a precise description, risk management enables the organization to handle all tasks in a responsible manner, cognizance of internal and external demands on the industry. The main principles as set out in Risk Management Standard AS/NZS ISO 31000 are briefly highlighted below. It is in order to state that risk management creates and protects value. Without risk management, the organization would encounter losses through various avenues including legal, security, and human health. When the organization applies risk management, it takes adequate measures to ensure that its objectives are achieved and losses minimized. Risk management is an important part of the organization’s activities and should be considered by all stakeholders in view of all processes. Without taking risk into account, decision makers would make choices without regard to important priorities. In view of risk management, mana gers and other decision makers cautiously undertake various activities and analyse various options before making choices. Uncertainty is one factor that risk management seeks to explicitly address. This is so considering that many threats and risks that a business encounters come unexpectedly and may bring massive losses to the organization. When risk management is done, the losses that may come with risks are minimized. The effectiveness of risk management depends on how it is done. When properly structured, and systematically and timely done, the results of the process are better compared to when otherwise done. Also, effective risk management involves the analysis of threats and risks based on available data and using the information available to predict possible outcomes of situations and their probabilities. However, in some cases, data is limited and decision makers have to stretch their minds in their risk management efforts. Risk management is not a standard procedure but de pends much on the internal and external environments of an organization. This means that the risk management activities of one organization may not effectively apply for another business. In addition, cultural and human factors must be taken into account if effective risk management has to be done. This is so considering that people working for the organization or those external to the organization can affect the business’

History of Twitter write a well researched paper on the evolution, Research

History of Twitter write a well researched on the evolution, current status and future trends for the New Media topic you have selected - Research Paper Example Barely into its fifth year, Twitter registered users are already currently estimated to be in the hundreds of millions and its revenues to be in the billions of dollars. Indeed, Twitter is a successful story worth telling and following. In 2006, a software engineer named Jack Dorsey approached Odeo, a media syndication company, to discuss his ideas of creating a â€Å"real-time status-communication platform with a social spin† where one could share and simply broadcast to others just about anything from gossip to simple thoughts. Biz Stone, the founder of Odeo, liked the idea and Dorsey’s proposal was declared as a side project of Odeo. It was decided that the SMS platform of mobile phones would be ideally suited for the project and a maximum of 140 characters, short of the 160 characters-limit of SMS text messaging, should be used as the broadcast limit (Thomases, p. x; Sagolla, p. xvii). Dorsey’s idea was a breath of fresh air to Odeo, which was at that time having a difficult time selling its latest technology Audio Blogger to users because of the stiff competition posed by such heavyweights as Apple Corp. The company decided it had to reinvent itself and Dorsey’s social networking microblogging idea was seen as an opportunity after it was presented in a day-long brainstorming of Odeo technocrats. Dorsey, Biz and Florian Weber went on to hammer out the prototype model of Dorsey’s proposal while the others continued with the usual company business to make sure that if Dorsey’s idea fails the company has something to fall back on (Sagolla p. xviii). The prototype was built in two weeks and the service was first tested by Odeo personnel and other insiders such as their immediate family members. It was entirely web-based and the first messages, which were broadcasted by Dorsey, were â€Å"waiting for dom to update